Equipment & Software Information

All equipment and software usage is restricted to undergraduate and graduate education. Equipment may NOT be used for research. Department shared research resources are available here.


Biological & Chemical

Analytical Balances

Analytical Balance (MS104S) by Mettler Toledo. Self-calibrating mass balances. Up to 7 significant figures. Capacity: 120 g. Minimum: 0.1 mg.

Links:
Quick Guide
Manual

Autopipettor / Pipette Filler

Autopipettors / Pipette filler by ThermoFisher. An automatic pipettor for macroscale liquid handling.

Links:
Manual

Bake Oven

Bake oven (Isotemp 500) by Fisher Scientific. A bake oven for the drying of glassware or for the curing steps of chemical reactions. Can also be used as an incubator, though it is not the intended use.

Links:
See instructions on machine.

Biosafety Cabinets

Biosafety Cabinet (SterilGard Advance III) by The Baker Company. For sterile culture of cells and tissues.

Links:

Centrifuge

Centra CL2 by Centra. A centrifuge with swinging-bucket rotor for the centrifugations of 15 mL and 50 mL centrifuge tubes. Four tube capacity. Speed Range: 1000-3175 RPM; Max RCF: 1750 x g; Max Capacity: 200 mL (4 x 50 mL).

Links:

Centrifuge (Micro)

Microcentrifuge (166-0602) by Bio-Rad. A microcentrifuge for the centrifugation of 1.5 - 2.0 µL samples. Has an attachment for the centrifugation of PCR tubes as well. Speed Range: 1000-14000 RPM.


Links:
Manuals


Centrifuge (Mini)

Minicentrifuge (166-0603) by Bio-Rad. A minicentrifuge for the centrifugation of 1.5 - 2.0 µL samples. Has attachments for the centrifugation of PCR tubes as well. Fixed speed: 6000 RPM.


Links:
See notes on machine.

CO2 Manifold

CO2 manifold (BIHL-4-2) by Western Innovator. A gas regulator for the Fisher Scientific 1325526 incubator ; this machine regulates the gas pressure from gas tanks in the main lab to create an atmosphere of specific composition in the incubator.

Conductivity Meters

Conductivity meter (Model 1056) by Amber Science. A device that measures the conductance of liquids pumped through it. Output is read in mV. Electrodes/probe/cell is Model 535.

Links:

Dry Bath

Single-block modular dry bath (Thermolyne Type 17600 Dri-Bath) by Barnstead International. Provides controlled source of dry heat for chemical and biological samples. 
Links:

Electrophoresis Equipment (E-Gel)

E-Gel Precast Agarose Electrophoresis System by Introgen. Used for quickly visualizing DNA using blue light instead of UV. Precast agarose gels are available (0.8% 16 well & 1.2% 12 well).

Links:
E-Gel Manual
E-Gel iBase Manual


Electrophoresis Equipment (Horizontal)

Horizontal electrophoresis equipment by Bio-Rad. Electrophoresis equipment for DNA, including PowerPac power supplies, Mini-Sub GT Gel-boxes and casting supplies with 8 and 15 well combs.

Electrophoresis Equipment (Vertical)

Vertical electrophoresis equipment by Bio-Rad. Electrophoresis equipment for protein, including PowerPac power supplies, Mini-Protean 2/3 Gel-boxes and casting supplies.


Links:
PowerPac Power Supply Manual
Mini-Protean 3 Manual with Sample Protocols


Fume Hoods

Fume hood (SafeAire) by Fisher Hamilton. Fume hood, for the handling of liquids or solids that produce noxious fumes or other phenomena hazardous to human health.

Links:

Hot Plates with Stirrers

Hot plates with a magnetic stirrers by Corning. Can be used to heat liquids and solids, or to make solutions. Model PC-620 with analog controls, Model PC-420D with digital controls.


Links:
Manual (Model PC-620)
Manual (Model PC-420D)


Ice Machine

Ice machine (QD-0132A) by Manitowoc. An ice machine for the constant generation of laboratory ice. NOT for consumption.

Links:
Manual


Incubator (CO2)

CO2 Incubator (Isotemp) by Fisher Scientific. An incubator intended for cell and tissue culture. Atmosphere composition and temperature can be controlled.


Links:
Manual


Incubator (Mini)

Mini-incubator (166-0501) by Bio-Rad. A compact 120V incubator for growing bacteria. Includes adjustable shelf, alcohol thermometer, and over-temperature protection. Capacity: eighty 6.5 cm culture plates. (Temperature Range: Room Temp to 60°C).

Links:
See notes on machine.

Microscopes (Inverted Light)

Inverted light microscope (Diaphot 300) by Nikon and (IMT-2) by Olympus. Can be used for the visualization of cells and other objects in the microscale visible spectrum. Imaging can be done with attached digital SLR camera on the Nikon microscope or using the SLR camera with the special adapter on the Olympus microscope.












Links:

Microwaves

Microwaves for the quick heating of liquids and gels, and for defrosting of samples of frozen substances. Not for food use.

Links:
Manual


Orbital Shakers

Digital orbital shaker (Sea Star) by Heathrow Scientific. A benchtop shaker for mixing solutions contained in laboratory vessels.
Links:

PCR Thermocycler

PCR Thermocycler (MyCycler) by Bio-Rad. A PCR machine, used to carry out polymerase chain reaction. Can be programmed to accommodate different PCR protocols. Allows temperature

Links:
Manual
Quick Guide
Specifications


Peristaltic Pumps

Peristaltic pump (Minipuls 3) by Gilson and (Dynamax) by Rainin. Peristaltic pumps for liquid handling. Pump heads can hold four tubes. Head speed is 0.01 to 48 rpm with flow rates of 0.3 μL/min to 40 mL/min.


Links:
Dynamax Manual
Minipuls 3 Manual


Peristaltic Pumps (Fisher)

Minipump by Fisher Scientific. A small bidirectional, self-priming peristaltic pump. Medium Flow Model (Operable Range: 0.4 to 85.0 mL/min).


Links:
Minipump Manual & Specs
Pump setup and Debugging Guide


pH Meter

pH Meters (AB15 & AR15/17) by Accumet. A device used to measure the pH of aqueous solutions.


Links:
Using the Accumet AR15 pH Meter in the lab
pH Meter Manual
Electrode Manual


Refrigerator & Freezer

Refrigerator and Freezer (Isotemp) by Fisher Scientific. A refrigerator designed for the storage of biological samples and reagents. Refrigerator operates at 4 °C, while Freezer operates at -20 °C.

Links:
Specifications


Scales / Mass balance

Mass balances/scales (CS2002) by Ohaus. A portable mass balance. Up to 3 significant figures. Capacity: 2000 g. Minimum: 1 g.


Links:
Using the Scale in Lab

Shaker Incubators

Orbital shaker incubators (MaxQ 4450) by Thermo Scientific. These incubators can be used for the culture of bacteria in liquid or solid media, during which agitation is required. Speed (Range: 15-500 RPM) and Temperature (Range: Room Temp.+ 5 °C - 80 °C) are user-controlled.

Links:
Manual


Spectrophotometer (Bio-Rad)

Spectrophotometer (SmartSpec Plus) by Bio-Rad. A versatile single-well spectrophotometer that can be used to take OD600 readings, Absorbance, UV, and many more. Absorbance λ Range: 200-800 nm.

Links:
Manual
Quick Start Guide
Specifications

Spectrophotometer (Vernier)

Spectrophotometer (SpectroVis Plus) by Vernier. Spectrophotometers for the measurement of properties of emission wavelengths from various samples. Absorbance λ Range: 380-950 nm. Can also measure fluorescence at 405 nm or 500 nm. LoggerPro Software required (available on all Lab PCs).

Links:
Using the SpectroVis and Software in the lab
Manufacturer's information and videos


Syringe Pumps

KDS 210 by KD Scientific. Pumps for the handling of automatic dispensation from syringes of various sizes.

Links:
Using the Syringe Pump in the lab
Manual

Transilluminator (UV)

UV Transilluminator (UV Transilluminator 2000) by Bio-Rad. A device that projects UV light through an object. Used primarily for the imaging of gels. UV shield included; all users must wear face masks!

Links:
Using the Bio-Rad UV Transilluminator in the lab
Manual

Transilluminator Imaging System (UV)

UV Transilluminator Imaging system (FOTO/Phoresis) by FOTODYNE, Inc. A digital camera hood packaged with a UV Transilluminator.

Links:
Using the Fotodyne UV Transilluminator Imaging System in the lab

Vortexers

Vortexers (BR2000) by Bio-Rad. A vortex designed for the mixing of liquids in 1.5 µL to 50 mL tubes.

Links:
See notes on machine.


Water Bath

Water baths (Isotemp 205) by Fisher Scientific. A water bath, used for the thawing of frozen substances, or for the regulation of temperature. The temperature range is from ambient to 60 °C with the cover open and ambient to 100 °C with the cover closed.

Links:
Manual




Electrical & Data Acquisition

Active Filter (UAF42)

Active Filter Integrated circuit (UAF42). It can create a series of different filter types.

Links:
Data Sheet
Application Note
Filter42 software (.exe file for 16 bit DOS machines or with an emulator)
Filter 42 Guide


Arduino Uno

Arduino Uno is an Arduino board which uses the Arduinio development platform. The lab has units that can be borrowed. Starter Kits are available for classes. The Arduino software is available at all of the lab computers.

Links:
Arduino Site
Starter Kit


Biopac Data Acquisition System & Sensors

Biopac Data acquisition system (MP35) & Sensors by Biopac. The physical interface between the BIOPAC software and sensors. Four channels and an electrode check are included. All lab PCs have the BIOPAC software installed. A number of sensors are available.

Links:
Available sensors in the lab
Using the Biopac software in the lab, sample.gtl file, sample.acq file
Hardware Manual
Student Manual
Student Tutorial

DAQ Boards (myDAQ)

Data Acquisition, or DAQ Boards, (myDAQ) by National Instruments. Used for interface between PCs (using either LabView, Matlab, or other software) and hardware/circuitry. Analog Input: +/-10V with a sampling rate of 200kS/s. Digital I/O are 3.3V LVTTL and are tolerant to 5V inputs. Digital output is not compatible with 5 V CMOS. Contains a +/-15V & +5V power source. In conjunction with the NI ELVISmx Software (available for free on the NI website), the device can be used as a Digital Multimeter, Function Generator, Bode Analyzer, Oscilloscope, etc. On BE lab PCs, the ELVISmx software can be accessed through Start >> All Programs >> BE ugrad lab software >> NI ELVISmx for myDAQ >> NI ELVISmx Instrument Launcher. 


Links:

NI myDAQ Support information with User Guides
Using the myDAQ as measurement and signal generation tools
Using the myDAQ and LabVIEW to control an LED
Device Manual
Electronics Tutorial
Matlab-myDAQ Interface Tutorial


DAQ Boards (USB-6008)

DAQ Boards (USB-6008) by National Instruments. A Data Acquisition, or DAQ Board. Used for interface between LabView software and hardware/circuitry.

Links:
Datasheet
Quick Start Guide
Manual


Electrical Test Cables

The BE labs have a variety of electrical test cables available. 

Links:
Electrical Test Cables


Function Generator / Signal Generator

Function/Signal Generator (AWG-4105) by Aktakom. An AC voltage generator, for applications in circuit design and analysis.

Links:
Manual

Multimeter

Multimeter (M-3800) by Metex. Multimeter can measure voltage, current, resistance, etc.

Links:
Manual

Oscilloscope

Oscilloscope (ADS-2022) by Aktakom. A device that can be used to observe electrical signals.

Links:
Manual

Oscilloscope/Signal Generator (Virtual)

Virtual Oscilloscope and signal generator (Stingray DS1M12) by USB Instruments. This device, along with the EasyScope software can operate as a signal generator and oscilloscope.

Links:
Using the device in the lab
Getting Started Guide
Manual

Power Supply (Variable DC)

VARIABLE DC power supply (9902PS) by MPJA. A single output DC power supply, for applications in circuit design and analysis. 120VAC input. Output: 3V, 4.5V, 6V, 7.5V, 9V, 12VDC @ 2000mA.


Power Supply (Variable DC, APS-7203)

Variable DC power supply (APS-7203) by Aktakom. A variable DC power supply with multiple outputs, for applications in circuit design and analysis.

Links:
Manual

Soldering Equipment (Aoyue)

Soldering Iron (INT9378) by Aoyue. A soldering iron; used to create permanent contacts between disparate electrical components. Fume extractors are available.

Links:
Manual


Mechanical

Drill (Battery-Powered)

Battery powered drill (DC930) by DeWalt. If you have never used a power drill before, please contact the lab staff before using.

Links:
Manual

Drill (Corded)

Corded power drill (D41) by Ryobi. If you have never used a power drill before, please contact the lab staff before using.

Links:
Manual

Instron Pneumatic Pump

Pneumatic pump by GDW Vacuum Technology, Inc. Used to provide pneumatic pressure for the Instron grips used in tensile testing. 30-45 psi is the safe range of activity for this pump.

Links:
Manual

Instron Test Frame (Model 4444)

Uniaxial mechanical tester (Model 4444) by Instron. Different attachments provide for tensile testing and 3-point bending. Connected to computer equipped with Bluehill interface software. Older model. Two systems are available, one with a 2KN load cell and the other with a 500N load cell. 

Links:
Using the Instron Test System in the Lab
Instron 4444 Reference Manual
Bluehill Software Manual

Instron Test Frame (Model 5544)

Uniaxial mechanical tester (Model 5544) by Instron. Different attachments provide for tensile testing and 3-point bending. Connected to computer equipped with Bluehill interface software. Newer model. Uses a 2KN load cell.

Links:
Using the Instron Test System in the Lab
Instron 5544 Reference Manual
Bluehill Software Manual

Laser Cutter

VLS 3.50 by Universal Laser Systems. Laser cutter for cutting sheets of materials. This device has a 50 W laser and bed size of 12" x 24". Users may only use materials provided by the lab (1/8" MDF and 1/8" & 1/4" acrylic). Access to the laser cutter is restricted to trained users (see training guide in Links below) and projects for Bioengineering classes which require laser cutting. Instructors should contact the Lab Staff to arrange use for their students.

Links:
Specifications
Laser Cutter Manual
Air Filter Manual
Laser Cut Thickness Guide

MakerCase: Easy Laser Cut Case Design (Creates SVG file)
Convert SVG files to DXF (can be read by 2D CAD Editor
Converting Solidworks to DWG for Laser cutting
Using the Laser cutter in the lab
Training procedure
Press fitting tutorial


Replicator 3D Printer

Replicator 3D printer (5th Generation) by MakerBot. Build volume: 11.2" x 6.0" x 6.1". Layer Resolution: High Accuracy: 100 µm (0.0039 in); Medium: 270 µm (0.0106in); Low Accuracy: 340 µm (0.0133 in). Material available: PLA (variable colors). All printing will be done by Lab Staff and is restricted to projects for Bioengineering classes which require 3D printing. Instructors should contact the Lab Staff to arrange use for their students.

Links:
Printing Instructions for the lab
Calendar of availability
MakerBot Replicator Manual
PLA Datasheet

Treadmill

Treadmill (390Pi) by Pro-Form. Used for human physiological studies.

Links:
Manual



Software & Other

Colony Count Matlab M-File

Bacteria colony counter program By Naveen Ponnappa, BE '12. A MATLAB m-file that assists with colony-counting on individual plates. Can be applied to numerous spot-counting situations. Instructions included in the m-file comments.

Links:
Colony Count m-file
YouTube Link


LabVIEW

LabVIEW programming language by National Instruments. Software package for rapid prototyping of data acquisition tools.


Links:
Introduction to LabVIEW site
LabVIEW Tutorial


Laser Printer

Color Laser printer (LaserJet CP4025) by HP.

Links:
Manual


LoggerPro

LoggerPro Software by Vernier. Software package for data analysis using Vernier tools. Bioengineering students can download the software by contacting the lab staff.

Links:
LoggerPro Software manual
LoggerPro website with tutorial videos

Matlab

Maltab by MathWorks. Software & Programming tool for Engineering, Math & Science.

Links:
Free Download for SEAS Students


Projector

Projector (NP2250) by NEC, Speaker system by JBL. A projector, linked to Station 12 in the main lab. Connections available for composite A/V or a separate laptop. The projector is connected to a speaker system. Instructions for using the projector in lab can be found at Station 12.


Links:
Manual

Serial Cloner

Free software tool for simulating molecular biology.

Links:
Software site


Wax Printer

Wax color printer (ColorQube 8570) by Xerox.

Links:
Manual